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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of the gene has long been observed in many tumors, which leads to chromosome instability, and therefore could contribute to tumorigenesis. This protein was uncovered to affiliate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in mobile-cycle controlled histone gene expression and performs a important function in endorsing mobile-cycle development inside the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, have been described. Two added splice variants had been noted but specific nucleotide sequence information just isn't still accessible.

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Blocking peptides are peptides that bind particularly to your target antibody and block antibody binding. These peptide generally contains the epitope acknowledged because of the antibody.

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Clicking the images or back links will redirect you to a web site hosted by BenchSci that gives 3rd-celebration scientific content. Neither the information nor the BenchSci engineering and processes for collection are actually evaluated by us; we are supplying them as-is and with out warranty of any type, such as to be used or software in the Thermo Fisher Scientific merchandise offered.

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This guideline outlines even further attainable optimization of cellular and immunoassay parameters to ensure the very best benefits are...

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This tutorial outlines further more possible optimization of cellular and immunoassay parameters to be sure the very best outcomes are...

Antibodies certain to the blocking peptide no more bind to your epitope to the focus on protein. By evaluating the staining through the blocked antibody compared to the antibody on your own, one can see which staining is specific.

Concentrate on Information and facts Cyclin E belongs into the remarkably conserved cyclin relatives, whose customers are characterized by a spectacular periodicity in protein abundance in the mobile cycle. Cyclins purpose as regulators of CDK kinases. Distinctive cyclins show distinctive expression and degradation patterns which lead for the temporal coordination of each mitotic party. Cyclin E varieties a complex with and capabilities for a regulatory subunit of CDK2, whose action is necessary for mobile cycle G1/S transition. This protein accumulates within the G1-S period boundary which is degraded as cells development through S phase.

The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by each antibodies...

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